Cellular Energetics - Fermentation & Cellular Respiration


Exercise 1 - Examination of yeast cells

Examine the yeast culture

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Yeast cells reproduce by a process known as budding, which is asexual. The basic division is by mitosis.


Exercise 2 - Carbon Dioxide Generation During Fermentation

From the overall steps of alcohol fermentation you can see that for every mole of glucose used, 2 moles of carbon dioxide are generated. One test for carbon dioxide is to bubble the gas in question through a barium hydroxide solution. The presence of a white precipitate indicates carbon dioxide gas since:

When the gas from an active yeast culture is bubbled through a 0.1 M Ba(OH)2 solution a white precipitate will form indicating the gas produced is carbon dioxide.


Exercise 3 - Benedict's Test for Reducing Sugars

Benedict's reagent is used as a simple test for reducing sugars. As stated in lab 2, reducing sugars have either a free aldehyde or a ketone group as part of their molecular structure.

Glucose is a reducing sugar, while the disaccharide sucrose is not.

Glucose heated in Benedict's reagent reduces Cu++ ions to form a green to brick-red precipitate depending on the amount of sugar present.

Five tubes were prepared:

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First published Sept 95: Modified Aug 05
Copyright © Michael Shaw 2005 (Images and Text)