PLNT3570
Fundamentals in Plant Pathology

 

Professor: Dr. Dilantha Fernando Tel: 474-6072 Room 205 Plant Science

E-Mail: D_Fernando@umanitoba.ca

 

Lab Instructor: Victoria Gauthier (Masters candidate) Tel: 474-6073 Plant Sci.

E-Mail: umgauthv@cc.umanitoba.ca

 

Lecture days and times: Tuesdays and Thursdays from 10.00 – 11.20 am
(1 h 20 min)

Room 218 Plant Science

Lab days and times: Wednesdays 2.30 – 5.30 pm Room 343 Faculty of Agriculture

This course is an introduction to the science of plant pathology. Topics include causal agents of plant diseases, symptoms and diagnoses, modes of infection and spread, effects of the environment on disease development, and methods of disease control. This course will offer a sound background for undergraduate students who may want to further their studies in plant pathology, molecular plant pathology, breeding or mycology majors, or do post graduate studies leading to Masters and Doctoral degrees. The students will be introduced to economically important diseases in the prairies they may encounter on crop plants and horticultural trees, the causal organisms, reproductive structures, and how environment and the host could contribute to the success of a pathogen and its infection process. The course will also cover plant’s defense mechanisms, and conventional and novel control strategies practiced in plant disease management with some emphasis on molecular tools that are presently in use to understand these mechanisms and phenomena.

 

Pre-requisite: BOTN 2210 (or 001.221) Biology of Fungi and Lichens

Class Material Covered:

  1. the importance of plant pathology in the prairies and cropping systems pathology
  2. definitions & common types of plant diseases
  3. influence of the host in disease
  4. influence of the pathogen in disease
  5. influence of the environment in disease
  6. causal agents of plant disease
  7. diseases caused by fungi
  8. diseases caused by bacteria
  9. diseases caused by viruses
  10. diseases caused by nematodes
  11. diseases caused by higher plants and abiotic factors
  12. plant disease management
  13. mycotoxins and their importance in disease
  14. novel disease management strategies (eg. Biological control)
  15. genetics of plant disease: host-pathogen interactions governed by genes
  16. Phytoalexins, chemical and structural defenses
  17. Hypersensitive Response (HR)
  18. Induced Systemic Resistance (ISR)
  19. Systemic Acquired resistance (SAE)
  20. Population genetics of pathogens: pathogen structure, variation and gene flow.

 

Text (Required): Plant Pathology (Fifth Edition) by G.N. Agrios (from BookStore)

Text (Optional): Diseases of Field Crops in Canada (Third Edition) by Bailey et al.

Journals: Phytopathology, Plant Disease, Can. J Plant Pathology, Plant Pathology

 

Examinations:

a) Mid-term exam.....20% (IN CLASS EXAM: to be determined)

b) Class participation...20%

b) Lab reports...........15%

c) Lab Final exam….15%

c) Final exam............30% (To be scheduled)

Class Participation (worth 20%): Each student will be given a topic to cover in a period of 1.5 hours (one lecture) in the early period of the course. The grade will depend on the effort the student has put to make a good presentation (power point), the content of the slides, and on the accuracy of the information. Students are encouraged to share other material related to their topic covered with the class. Each student should bring over the powerpoint presentation to the professor at least one week before class so he can go through and see if the material is appropriate and change/add if necessary. Students are also encouraged to give a few important examples of human pathogens that belong to the organisms that they cover, so we see parallels/differences in human diseases and plant diseases. The topics will be:

  1. Bacteria, mollicutes, mycoplasma / phytoplasma
  2. Viruses, viroids, and virus-like organisms
  3. Nematodes and protozoa
  4. water molds, oomycetes, chytridiomycetes
  5. Ascomycetes and Deuteromycetes
  6. Basidiomycetes and parasitic higher plants

 

We will have a “lottery Draw” to select and assign the topics.

 

Missing mid-term and/or lab assignments and exam:

A grade of 0 (zero) will be assigned to any student who misses an exam without a valid reason or without the consent of the professor (all exams) and instructor (lab assignments). See detailed description on how and when lab assignments should be handed (in lab outline). No rescheduling of an examination will be allowed, regardless of the circumstances. Those who miss the examination (with a valid reason) will have their final exam accounting for 30% (final exam) + the value of the missed examinations.

 

Plagiarism and cheating: Refer to the University of Manitoba General Calendar regarding the definition and penalties associated with impersonations, cheating and plagiarism.

 

Feedback: Feedback will be given to students following the mid-term exam and prior to the voluntary withdrawal of March 19, 2009.

 

 

 

PLNT3570
Fundamentals in Plant Pathology (Lab Session)

 

Laboratory schedule*

 

Lab

Topics Covered

Date

1

Tour of the plant pathology lab

Sterilization of apparatus; culture media

Identification of plant diseases (Pt I)

Jan 14

2

Identification of plant diseases (Pt II)

Culture media preparation

Jan 21

3

Culture of pathogens and isolation

Jan 28

4

Identification of pathogens through the microscope: structure and spore types

Feb 4

5

Chemical control: types of chemicals, methods of application and safety

Biological control (fungi and bacteria)

Lecture on Viruses (Dr. Steve Haber, AAFC Winnipeg)

Feb 11

-

READING WEEK

Feb 18

6

Identification of plant diseases through inoculations (Koch’s postulates)

Feb 25

7

Identification of plant pathogens through molecular methods

Mar 4

8

Breeding for disease resistance: inoculation methods and screening

Mar 11

9

Lecture on Nematodes (Dr. Mario Tenuta, Dept. of Soil Science)

Mar 18

 

*The schedule will be followed as much as possible. However, this schedule is not set in stone.