University of Manitoba - Development & Advancement - Histological and Histochemical Stains
Histological and Histochemical Stains

 

STAIN

RESULTS

SPECIFICITY / TISSUE

PRICE PER SLIDE

IMAGE(S)

 

 

 

 

 

 

Alcian blue

(pH 2.5)

Strongly acidic sulfated mucosubstances

Blue

Intestine

Appendix

Colon

$1.50

 

Nuclei

Pink/Red

Cytoplasm

Pale Pink

 

 

 

 

 

 

Alcian blue &

Periodic acid Schiff

Acid mucins

Blue

Intestine

Appendix

Colon

$2.50

 

Image 1 (20x)

Image 2 (40x)

Neutral mucins

Magenta

Mixtures

Blue/Purple

Nuclei

Deep blue

 

 

 

 

 

 

Hematoxylin & Eosin

Collagen

Pale pink

All tissues

$1.00

Image 1 (20x)

Image 2 (40x)

Muscle

Deep pink

Acidophilic cytoplasm

Red

Basophilic cytoplasm

Purple

Nuclei

Blue

Erythrocytes

Cherry red

 

 

 

 

 

 

Masson Trichrome

Cytoplasm

Red

All tissues

$5.00

Image 1 (20x)

Image 2 (40x)

 

Keration

Red

Muscle fibres

Red

Nuclei

Black

Collagen

Blue

Mucins

Blue

 

 

 

 

 

 

Nissl/Cresyl violet

Neruron (Nissl body)

Pink/Violet

Nervous system

Brain

Spinal cord

$2.50

Image 1 (20x)

Image 2 (40x) 

 

 

 

 

 

 

 

Nuclear fast red (Counter stain)

Nuclei

Red

All tissues

$1.50

Image 1 (20x)

Image 2 (40x)

 

 

 

 

 

 

Oil red O

Lipids

Bright Red

Cryosections of adipocytes

$3.00

Image 1 (20x)

Image 2 (40x)

Nuclei

Pale blue

 

 

 

 

 

 

Picrosirius red

Nuclei

Black

Grey

Brown

Collagen

$2.50

Image 1 (20x)

Image 2 (40x)

 

 

Collagen

Red

Background

Yellow

 

 

 

 

 

 

Prussian blue

Ferric salts

Deep blue

Ferric ions

Brain

Bone marrow

Blood

$2.50

 

Image 1 (20x)

Image 2 (40x)

 

Nuclei

Red

Erythrocytes

Yellow

 

 

 

 

 

 

Periodic acid Schiff

Neutral mucins

Magenta

Intestine (goblet, paneth cells)

Mucous gland

$2.00

Image 1 (20x)

Image 2 (40x)

 

 

 

 

 

 

 

Safranin O

Nuclei

Black

Cartilage

$3.00

Image 1 (40x)

Cytoplasm Cartilage

Gray green

Mucin,

Mast cell granules

Orange to red

 

 

 

 

 

 

Toluidine blue

Mast cell granules

Red/Purple

Mast cells

Nuclei

$1.50

Image 1 (40x)

Nuclei

Dark blue

Background

Light blue

 

 

 

 

 

 

Verhoeff van Gieson

Elastic fibers

Blue/Black

Elastic fibers

Aorta

Kidney

Myometrium

$3.00

Image 1 (40x)

Image 2 (40x)

 

Nuclei

Blue/Black

Collagen

Red

Muscle and other tissue elements

Yellow

Prices are subject to change due to increases in chemical costs.
Additional costs may also apply for optimization of personalized special requests.

Immunohistochemistry & Immunofluorescence:

Please contact our Histological Technicians at histology_services@umanitoba.ca for special staining requests and prices:

Immunohistochemistry and immunofluorescence will be a custom request for each client.  The client is required to provide all sample material including any necessary positive and negative controls.  Primary and secondary Antibodies, IgG controls, normal serum, blocking solutions, nuclear stains excluding hematoxylin or DAPI, buffers except TBS and PBS and any developing substrates, kits or complexes must be provided.  If all supplies are not provided, the histology lab will order what is required with a supplied FOAP or account number. 

A pilot experiment will be designed with histology staff and the client and only carried out once the protocol is agreed upon.  The client will then assess the slides to determine any necessary changes to the protocol.  Multiple pilot experiments may be conducted before proceeding with the desired samples.  Only once the client approves the pilot will the full run of samples be completed.  Any analysis of the final product will be done by the client and NOT histology staff.

Each pilot and experimental run will be charged $25.00/hour (approximately 7 hours depending on slight protocol changes and the number of slides) as well as $30.00/slide.