|
Service |
Description |
Chemical(s) Used |
Cost For Service |
|
Processing Tissue for Paraffin |
Placing tissue that arrives in fixation (already grossed) into cassettes and labeling for the tissue processor |
Fixative, Cassettes Processor runs overnight using Formalin, Dehydrating Alcohol, Xylene and Paraffin. |
1-24 cassettes - $25 25-50 = $55 51-75 = $80 76-100 = $100 ($2.50 per cassette) ($25/hour labour) |
|
Embedding Tissue in Paraffin |
Taking the cassettes from the tissue processor and setting them in wax in the correct orientation, then filling the mold with wax to create the block |
Wax from the Embedding machine |
Tissue processing included ($2 each, if separate) |
|
Cutting Paraffin Sections |
Cutting the desired number of sections from each block |
Knife blades (one for every 3-5 blocks) |
$25/hour labour $0.50/slide (plain) $0.75/slide (APTEX) $1.00/slide (Plus) |
|
Staining Paraffin Sections |
Staining and cover slipping slides |
Depends on the stain |
$25/hour labour $1/slide (H&E) |
|
Imaging |
Image the slides using the light of fluorescent microscope and taking the requested pictures at each magnification |
n/a |
$25/hour labour |
|
CD/Disc |
Upload, transfer and save the images to a CD/Disc |
n/a |
$5 per disc |
Parrafin Sample Preparation Guidelines:
For ideal sample processing, tissues must be properly prepared. Please prepare your tissue as follows (contact Histology Lab staff with any questions):
Size - Samples should be grossed to a reasonable size before fixation:
- Grossed slices no thicker than 5mm and a maximum of 1.5cm length and width. Smaller pieces have better fixation and are easier to section.
- Provide only tissue significant to your research, having grossed out any other attached tissue or organs that are not necessary.
- If you deal with larger specimens, please provide only a biopsy.
Fixation - please fix all tissues in appropriate fixative based on what the samples are to be used for. Some fixatives may interfere with staining or immunohistochemistry conditions. Common fixatives used are 10% Buffered Formalin or 4% Formaldehyde (prepared from a 37% stock liquid containing methanol commercially purchased or prepared from a powder).
Minimum fixation time should be 24 hours and can last as long as 5 days.
- Your tissue sample should be free floating in fixative of at least 10X the tissue volume.
Once your samples are in fixative, they may be delivered to the Histology Lab at a pre-arranged appointment time with the completed Service Request Form. Please bring a slide box of sufficient size for the requested service at the time of sample drop off.