IMMU7040 - Immunological Methodology
January 14 - April 29, 2014
CRN #24644 (A01)
CRN #24644 (A01)
| Course Co-ordinator: | Dr. Aaron Marshall |
| Office: | 415 Apotex Centre |
| Phone: | (204) 789-3385 |
| Email: | Aaron.Marshall@med.umanitoba.ca |
| Course Times: | Tuesdays, 9:30-11:30 a.m. (Some lab components may run on the Tuesday afternoon on the day of the lecture.) |
| Location: | Alec Sehon Seminar Room 477 Apotex Centre 4th Floor, Department of Immunology |
Description:
A practical course covering a range of methodologies in common use in Immunology and other biomedical research fields.
Lectures will cover:
- theoretical basis of methods
- common applications in immunology
- practical pitfalls and common problems
- advantages and disadvantages of competing methodologies
Exam Format: Take-home exam of experimental problems incorporating several of the covered methodologies. Answers independently graded by at least 3 instructors and averaged.
Evaluation:
| Mid-term exam (take-home): | 30% |
| Final Exam (take-home) | 70% |
Class Schedule:
See link below to download class notes from the password protected website. The password will be distributed at the first class.
| Date | Topic | Lecturer |
| Jan. 14 | Introduction to Methodology/Leukocyte purification methods | A. Marshall |
| Jan. 21 | Methods for generation of antibodies binding a specific molecular target *** Cangene Ab production lab tour - to be confirmed |
J. Antony George |
| Jan. 28 | Detecting and measuring protein interactions | N. Mookherjee |
|
Feb. 4 |
Immunoprecipitation and electrophoretic analysis of proteins | S. Gibson |
| Feb. 11 | Enzyme immunoassays and their applications | R. Su |
| Feb. 18 | Microscopy techniques: Immunohistology, immunofluorescence and confocal microscopy ***Lab demo to follow 12:30-1:30 p.m. |
A. Marshall |
| Feb. 25 | Flow cytometry ***Lab demo to follow 12:30-1:30 p.m. |
A. Marshall |
| Mar. 4 | RNA work, RT-PCR and quantitative analysis of gene expression ***Lab demo to follow lecture MIDTERM EXAMINATION The exam will cover lectures 1-7 and is worth 30% of your final grade. It will be handed out after class. It is due at 4:00 p.m., March 7, 2014. |
A. Soussi Gounni |
| Mar. 11 |
DNA manipulation, expression vectors, gene transfer methods |
S. Kung |
| Mar. 18 | Functional analysis of genes in cells and organisms | A. Marshall |
| Mar. 25 | Gene microarrays: practical aspects and data analysis | A. Raouf |
| April 1 | Proteomics/high content analysis ***Tour/demo at Manitoba Centre for Proteomics - to be confirmed |
J. Wilkins |
| April 8 | Animal models for host-defense against pathogens | J. Uzonna |
| April 15 | Animal models for inflammatory disease | J. Ghia |
| April 22 | Methodological issues in measuring immune responses in human populations | J. Rempel |
| April 29 | Pick up FINAL EXAM in Immunology Office, due May 5 before 4 p.m. |