Dr. John L. Wylie
Assistant Professor, Medical Microbiology
Assistant Professor, Community Health Sciences

Degrees: M.Sc. (Ottawa, 1989), Ph.D. (Manitoba, 1994)

Mailing Address: Cadham Provincial Laboratory
P.O. Box 8450
750 William Ave.
Winnipeg, Manitoba
R3C 3Y1

Tel: 204-945-7473 Fax: 204-786-4770 Lab: 204-945-7311
E-mail: john.wylie@gov.mb.ca
Research Interests:
My research interests focus on the epidemiology of bacterial and viral pathogens. The primary goals of my research are to determine the transmission dynamics of these pathogens within groups or populations of people and to translate that research into information useable by public health departments to improve or plan new public health control programs. The primary tools used in my lab are molecular typing techniques to identify individual strains of a bacterial or viral pathogen; geographic information system analysis to track the movement of these strains; and questionnaire based interviewing of members of high risk groups to identify the risk factors associated with infection and transmission of different pathogens. Interviews are designed to gather data on both individual-based behaviours and social network relationships. Social network analysis studies the interactions between people and is useful for analyzing the movement of pathogens through groups of people. This latter analysis provides the social context associated with disease acquisition. Current and past projects have dealt with Chlamydia trachomatis, Neisseria gonorrhea, HIV, hepatitis C, hepatitis B, and enteric bacteria.


Recent Publications:
  1. Gilmour, M. W., T. Cote, J. Munro, L. Chui, J. L. Wylie, J. Isaac-Renton and L. Ng. 2005. Multi-locus sequence typing of stx+ Escherichia coli O26:H11 isolates in Canada does not identify genetic diversity. J. Clin. Microbiol. (in press)
  2. Wylie, J. L., and D. L. Nowicki. 2004. Community- and Healthcare-acquired Methicillin-resistant Staphylococcus aureus in Manitoba, Canada. J. Clin. Microbiol. (in press).
  3. Wylie, J. L., T. Cabral, and A. M. Jolly. 2004. Identifying STI transmission networks: a molecular, geographic, and social network analysis. J. Infect. Dis. 191(6): 899-906.
  4. Lai-King, N., Martin, I., Lau, A. and the National Gonococcal Surveillance Program Members (Shaw, C., Tyrrel, G., Wylie, J. L., Jamieson, F., Brown, S., Turcotte, P., Forward, K., Hardy, G. J., Abbott, L., and Ratnam, S). 2003. Trends of chromosomally mediated antimicrobial resistance in Neisseria gonorrhoeae in Canada. Sex. Trans. Dis. 30:896-900.
  5. Cabral, T., A. Jolly, and J. L. Wylie. 2002. Genotypic diversity of Chlamydia trachomatis and concordance with sexual network data. J. Infect. Diseases. 187: 279-286.
  6. Macey, J., A. Johnston, L. Elliott, D. Krahn, D. Nowicki, J. Wylie. 2002. Outbreak of diarrhoeal illness in attendees at a Ukrainain dance festival, Dauphin, Manitoba - May, 2001. Can. Commun. Dis. Report. 28:141-145.
  7. A. M. Jolly and J. L. Wylie. 2002. Gonorrhoea and chlamydia core groups and sexual networks in Manitoba. Sex. Trans. Infect. 78(Suppl I):1145-1151. Conference proceedings: Phase specific strategies for the prevention, control, and elimination of sexually transmitted diseases, Rome, Italy.
  8. Boyd, D., T. Cabral, P. Van Caeseele, J. L. Wylie, and M. R. Mulvey. 2002. Molecular characterization of the vanE gene cluster in vancomycin-resistant Enterococcus faecalis N00-410 isolated in Canada. Antimicrob. Agents Chemother. 46:1977-1979.
  9. Embil, J. M., M. Almuneef, D. Nicoll, S. Makki, G. Cunningham, J. L. Wylie, L. Nicolle, Z. A. Memish. 2001. Methicillin-resistant Staphylococcus aureus: profiles oceans apart, comparing a Canadian and Saudi Arabian Experience. J. Chemother. 13, Suppl. 1:28-33.
  10. Jolly, A. M., S. A. Muth, J. L. Wylie, J. Potterat. 2001. Sexual networks and sexually transmitted infections; a tale of two cities. J. Urban Health. 78:433-445.
  11. Van Caeseele, P., S. Giercke, J. L. Wylie, S. Amin, M. Ofner-Agostini, D. Boyd, M. Mulvey. 2001. Identification of the first Enterococcus faecalis harbouring vanE in Canada. Can. Commun. Dis. Report. 27:101-104.
  12. A. M. Jolly and J. L. Wylie. 2001. Sampling individuals within large sexual networks: an evaluation of four approaches. Sex. Trans. Dis. 28:200-207.
Laboratory Members:
Lena Shah MSc student, Community Health Sciences  
Pat Sulaiman MSc student, Community Health Sciences  
Stephanie Harvard MSc student, Community Health Sciences  
Laura Thompson MSc student, Community Health Sciences