Viral Immunology Core
 
Location:
Room 516 Basic Medical Sciences Building
Service Director:
Dr. Keith Fowke

Facility History:

The Viral Immunology Core was developed to facilitate studies in the interaction between viral pathogens and the immune system. The Core consists of a Biosafety Level (BSL) 3 suite, designed for the propagation of HIV, and an adjoining BSL 2 suite utilizing BSL 3 practices (eg. double glove and gown) for culturing BSL 2 such as Epstein Barr Virus (EBV), Hepatitis C Virus (HCV), and Human Herpesvirus Type 6 (HHV6). In addition to viral isolations cultures the lab is able to perform assays such as ELISpots, cell proliferations, ELISAs, genomic isolations, DNA quantitations, fluorescent and digital microscopy, luminescence detection, specimen cryopreservation and storage, and gradient gene amplification.

Facility Equipment:
The Core is equipped with four six-foot Biological Safety Cabinets, six CO2 incubators, a Packard cell harvester, a Packard Topcount 96 well scintillation and luminescence detector, a Molecular Devices Spectramax UV spectrophotometer/ELISA reader, an Eppendorf Mastercycler gradient thermal cycler, an MVE 1520 HE 33,000 sample liquid nitrogen storage facility, an ELISpot reader, and a Nikon inverted fluorescent/phase contrast microscope with digital camera. The lab is networked to a server so that data can be easily and safely exported out of the laboratory.

Services Provided:
Work is performed on a collaborative or contract basis. A laboratory safety training program has been established that includes video and in-lab training and a testing element.

How to get services:
For more information contact Dr. Keith Fowke at 787-3818.

Cost of services:
Costs are project dependent.