Dr. Sabine Mai

Professor Physiology, Biochemistry & Medical Genetics, Human Anatomy and Cell Science
Director of The Genomic Centre for Cancer Research and Diagnosis (GCCRD)[Basic Research]

Degrees:
B.Sc. in Biology, Univ. of Cologne, Germany, 1981
M.Sc. in Biology, French & Pedogogics, Univ. of Cologne, Germany, 1985
Ph. D. in Molecular Biology, Univ of Karlsruhe, Germany, 1991

Mailing Address:
Manitoba Institute of Cell Biology
675 McDermot Ave. Rm. ON6046
Winnipeg, MB R3E 0V9

Tel: (204) 787-2135      Fax: 787-2190     Lab: 787-4125
E-mail: smai@cc.umanitoba.ca

The Genomic Centre for Cancer Research and Diagnosis (GCCRD) is 10 years old.
 Click here to view pictures from our Imaging Symposium on June 5, 2009 celebrating this event. 

Research Interests:

• Mechanisms of c-Myc-dependent genomic instability. Analysis of replication- and/or segregation-driven mechanisms of c-Myc-dependent gene amplification.
• Myc mutants in genomic instability, apoptosis, and tumor development
• Structure and function of c-myc induced extrachromosomal elements (EEs).
• Genomic instability in pre-malignant cells. Tumor initiation and progression.
• The three-dimensional organization of the interphase nucleus in normal, immortalized and tumor cells.
  (Sample Movie 1) 
  (Sample Movie 2)
  
  Figure legends for movies. The 3D movies show the positions of chromosomes 5 (red) and 13 (green) in a diploid mouse Pre B lymphocyte line. Note the movement of both chromosomes 5 and 13 thirty hours after experimentally induced c-Myc deregulation. For details, see Louis et al., 2005.

Training opportunities / workshops and courses offered

"Innovative Technologies in Multidisciplinary Health Research Training". Advanced molecular imaging and analysis and applied genomics using emerging innovative technologies. Multinational, multidisciplinary, and industry-involved. Training target groups: graduate students, medical students, MD/PhD students, residents, clinical fellows, clinician-scientists, visiting fellows, clinical geneticists, technicians and research associates. Training through workshops, seminars, short- and long-term research projects. For details, see www.itmhrt.ca

Focus on mechanisms of genomic instability.

Genomic instability is a hallmark of pre-neoplastic and neoplastic cells. The mechanisms underlying the induction of genomic instability in cells have been poorly elucidated. We were first to demonstrate that c-Myc deregulation induces locus-specific genomic instability (Mai, 1994) and karyotypic instability (Mai et al., 1996a). Using c-Myc-inducible cell lines, we have identified the following genes as targets of c-Myc in genomic instability: dihydrofolate reductase (DHFR) (Mai, 1994, 1996b, Taylor et al., 1997, Taylor and Mai, 1998), ribonucleotide reductase R2 (Kuschak et al., 1999a), cyclin D2 (CCND2) (Mai et al., 1999), ornithine decarboxylase (ODC) (Smith et al., 2002, 2003), cyclin B1 (Mai et al., 2004). For reviews, see Mai and Mushinski, 2003; Kuttler and Mai, 2005. Our ongoing work focuses on the initiating mechanisms of c-Myc-mediated genomic instability and on the three-dimensional organization of the nucleus. The following research areas are actively pursued:

1) c-Myc-induced illegitimate DNA replication and re-organization of the genome.
2) c-Myc and mutant myc box II Myc protein-induced genomic instability and apoptosis.
3) c-Myc induced formation of extrachromosomal elements (EEs). 
4) Mechanisms of plasmacytoma development. 
5) The three-dimensional organization of the mammalian nucleus. 

Our previous work on c-Myc-dependent genomic instability.

c-Myc-dependent genomic instability has been observed in cell lines of different species and tissue origins, and also in vivo. DHFR gene amplification was found within the first week of pristane-induced c-Myc-dependent plasmacytomas in Balb/c mice. Moreover, we observed the spontaneous c-Myc deregulation in 5-15% of all hematopoietic cells of the fetal liver of p53 nullizygous (p53-/-) embryos as well as in the bone marrow, spleen, thymus, and primary fibroblasts of 4-6 week old p53 nullizygous (p53-/-) mice. The deregulation of c-Myc was associated with the locus-specific amplification of c-Myc target genes. In contrast, age-matched control mice (p53+/+) did not display genomic instability and c-Myc deregulation.

Mechanisms of c-Myc deregulation in translocation-negative plasmacytomas.

The activation of the c-myc gene is key to the development of all murine plasmacytomas (PCTs), resulting in deregulated levels of endogenous c-Myc protein expression. In the majority of pristane-induced mouse PCTs, the deregulation of c-myc transcription is achieved by chromosomal translocation that juxtaposes the c-myc/pvt-1 locus on chromosome 15 to one of the immunoglobulin (Ig) loci: on chromosome 12 (IgH), 6(IgK) or 16(IgL).

Several translocation-negative PCTs with deregulated Myc expression have been described. The histological features of such PCTs and the course of disease are indistinguishable from the ones with the typical chromosomal translocations. We have recently studied a translocation-negative mouse plasmacytoma, DCPC21, in great detail (Wiener et al., Proc. Natl. Acad. Sci. (USA) 96: 13967-13972, 1999) (see Fig.1). We have found that c-myc is juxtaposed to IgH on extrachromosomal elements (EEs). These EEs are functional genetic units able to transcribe c-myc as shown in mRNA track studies on the single cell level. This is further supported by the presence of active chromatin on these extrachromosomal DNA molecules. We have shown in addition, that the c-myc/IgH-containing EEs are able to confer c-Myc expression to non-proliferating B cells upon extrachromosomal gene transfer. Interestingly, when introduced into primary B cells, the c-myc/IgH-carrying EEs induce cell death in the majority of the target cells within 24 hours.


Figure 1

Development of novel technologies to study genomic instability.

- Quantitative analysis of protein levels and gene copy numbers by fluorescent in situ hybridization (FISH) within the same cell. This assay is called CPFA (Combined Protein/FISH Analysis)(Fukasawa et al. Oncogene 15: 1295-1302. 1997)..
- Analysis of the total cellular population of extrachromosomal elements (EEs) by FISH. This assay allows one to examine every gene of choice for its presence on EEs (Kuschak et al. Technical Tips Online. T01669. 1999).
- Banding technology for mouse chromosomes that works after spectral karyotyping (SKY). This allows one to examine mouse chromosomes as carefully as human chromosomes in SKY analyses (Wiener and Mai. Technical Tips Online T01884. 2000).
- Protocol for the preparation of elongated mouse chromosomes.
- Three-dimensional imaging of the interphase nucleus.
-Multicolor banding (mBAND) of mouse chromosome 11


Recent Publications:

1. Chen Q, Shi X, Rudolph C, Yu Y, Zhang D, Zhao X, Mai S, Wang G, Schlegelberger B, Shi Q. Recurrent trisomy and Robertsonian translocation of chromosome 14 in murine iPS cell lines. Chromosome Res. 2011 Oct 19. [Epub ahead of print]
2. Gadji M, Vallente R, Klewes L, Righolt C, Wark L, Kongruttanachok N, Knecht H, Mai S. Nuclear remodeling as a mechanism for genomic instability in cancer. Adv Cancer Res. 2011; 112:77-126. Article and cover. 
3. Dawson AJ, Bal S, McTavish B, Tomiuk M, Schroedter I, Arshad N. Ahsanuddin AN, Seftel MD, Vallente R, Mai S, Cotter PD, Hovanes K, Gorre M, Gunn SR. Inversion and deletion of 16q22 defined by array CGH, FISH, and RT-PCR in a patient with AML. Cancer Genetics 204 (2011) 344e347.
4. Righolt C, Wiener F, Taylor-Kashton C, Harizanova J, Vermolen B, Garini Y, Young IT, Mai S. Translocation frequencies and chromosomal proximity for selected mouse chromosomes in primary mouse B lymphocytes. Cytometry Part A. 79A: 276-283, 2011.
5. Scaltriti M, Eichhorn PJA, Cortés J, Prudkin L, Aura C, Jiménez J, Chandarlapaty S, Serra V, Prat A, Ibrahim YH, Guzmán M, Gili M, Rodríguez O, Rodríguez S, Pérez J, Green SR, Mai S, Rosen N, Hudis C and Baselga J. Cyclin E amplification/overexpression is a mechanism of trastuzumab resistance in HER2 positive breast cancer patients. Proc Natl Acad Sci (USA). 2011 Mar;108(9): 3761-66. Feb 14. [Epub ahead of print]
6. Klewes L, Höbsch C, Katzir, N, Rourke D, Garini Y, Mai S. Novel automated three-dimensional (3D) genome scanning based on the nuclear architecture of telomeres. Cytometry Part A. 2011 Feb;79(2):159-66. doi: 10.1002/cyto.a.21012. Epub 2010 Dec 30.
7. Knecht H, Mai S. 3D imaging of telomeres and nuclear architecture: an emerging tool of 3D nano-morphology based diagnosis. J Cell Physiol. 2011 Apr;226(4):859-67.
8. Dawson AJ, Yanofsky R, Vallente R, Bal S, Schroedter I, Liang L and Mai S. Application of microarrays to the cytogenetic analysis of paediatric acute lymphoblastic leukemias. Current Oncology 2011 Oct;18(5):e210-7.
9. Knecht H, Brüderlein S, Wegener S, Lichtensztejn D, Lichtensztejn Z, Möller P and Mai S. 3D nuclear organization of telomeres in the Hodgkin cell lines U-HO1 and U-HO1-PTPN1: PTPN1 expression prevents the formation of very short telomeres including “t-stumps”. BMC Cell Biology. 2010 Dec 14;11(1):99. [Epub ahead of print] BMC Cell Biology cover image of the month.
10. Rio Frio T, Lavoie J, Hamel N, Geyer FC, Kushner YB, Novak DJ, Wark L, Capelli C, Reis-Filho JS, Mai S, Pastinen T, Tischkowitz MD, Marcus VA, Foulkes WD. Homozygous BUB1B Mutation and Susceptibility to Gastrointestinal Neoplasia. N Engl J Med. 2010 Dec 30;363(27):2628-2637.
11. Guffei A, Sarkar R, Klewes R, Righolt C, Knecht H, Mai S. Dynamic chromosomal rearrangements in Hodgkin’s lymphoma are due to ongoing 3D nuclear remodeling and breakage-bridge-fusions. Haematologica. 2010 Dec;95(12):2038-46. Epub 2010 Sep 7.
12. Wiener F, Schmälter A-K, Mowat MRA, Mai S. Duplication of sub-cytoband 11E2 of chromosome 11 is always associated with accelerated tumor development in v-abl/myc induced mouse plasmacytomas. Genes & Cancer 1(8): 847-858. 2010. Article and journal cover. 
13. Klonisch K, Wark L, Hombach-Klonisch S and Mai S. Nuclear imaging in three dimensions: A unique tool in cancer research. Ann Anat. 2010 Sep 20;192(5):292-301. Epub 2010 Aug 6. Article and journal cover.
14. Knecht H, Brüderlein S, Mai S, Möller P, Sawan B. 3D structural and functional characterization of the transition from Hodgkin to Reed-Sternberg cells. Ann Anat. 2010 Sep 20;192(5):302-8. Epub 2010 Aug 6. Article and journal cover.
15. Millau J-F, Mai S, Bastien N, Drouin R. p53 functions and cell lines: have we learned the lessons from the past? BioEssays 32: 392-400. 2010
16. Mai S. Initiation of telomere-mediated chromosomal rearrangements in cancer. Prospects article. J Cell Biochem. 109: 1095-1102. 2010. Featured article.
17. Silva AGdS, Graves HA, Guffei A, Ricca TI, Mortara, RA, Jasiulionis MG, Mai S. Telomere-centromere-driven genomic instability contributes to karyotype evolution in a mouse model of melanoma. Neoplasia 12 (1): 11-19. 2010.
18. Millau J-F, Mai S, Bastien N, Drouin R: p53 functions and cell lines: have we learned the lessons from the past? BioEssays 32: 392-400. 2010.
19. Silva AGdS, Graves HA, Guffei A, Ricca TI, Mortara, RA, Jasiulionis MG, Mai S. Telomere-centromere-driven genomic instability contributes to karyotype evolution in a mouse model of melanoma. Neoplasia 12 (1): 11-19. 2010.
20. Mai S. Initiation of telomere-mediated chromosomal rearrangements in cancer. Prospects article. J Cell Biochem. 109: 1095-1102. 2010. Featured article.
21. Gadji M, Fortin D, Tsanaclis A-M, Garini Y, Katzir N, Wienburg Y, Yan J, Klewes L, Klonisch T, Drouin R, Mai S*. Three-dimensional (3D) nuclear telomere architecture is associated with differential time to progression and overall survival in glioblastoma patients. Neoplasia. 12 (2): 183-191 2020. 
22. Knecht H, Sawan B, Lichtensztejn Z, Lichtenstejn D, Mai S. 3D Telomere FISH defines LMP1 expressing Reed-Sternberg Cells as End-Stage Cells with Telomere-poor Ghost Nuclei and very short Telomeres. Lab Invest. 2010 Apr;90(4):611-9. Epub 2010 Feb 8.
23. Davie JR, Drobic B, Perez-Cadahia B, He S, Espino PS, Sun JM, Chen HY, Dunn KL, Wark L, Mai S, Khan DH, Davie SN, Lu S, Peltier CP, Delcuve GP. Nucleosomal response, immediate-early gene expression and cell transformation. Adv Enzyme Regul. 2009 Nov 4. [Epub ahead of print]
24. Gadji M, Crous AM, Fortin D, Krcek J, Torchia M, Mai S, Drouin R, Klonisch T. EGF receptor inhibitors in the treatment of glioblastoma multiform: Old clinical allies and newly emerging therapeutic concepts. Eur J Pharmacol. 2009 Oct 18. [Epub ahead of print]
25. S. Lacoste, E. Wiechec, G. Williams, M. Henriksson, G. Klein, Mai S. Chromosomal rearrangements after ex-vivo Epstein-Barr virus (EBV) infection of human B cells. Oncogene 2010. Jan 28:29(4):503-15. Epub 2009 Nov 2.
26. Gonzalez-Suarez I, Redwood AB, Vermolen B, Lichtensztejin D, Bhat A, Sullivan T, Sage J, Stewart CL., Mai S and Gonzalo S. A-type lamins constitute a higher level of regulation of telomere biology. EMBO J. Aug 19;28(16):2414-27. 2009. Epub 2009 Jul 23.
27. I. Bronstein, Y. Israel, E. Kepten, S. Mai, Y. Shav-Tal, E. Barkai, Y. Garini. Transient anomalous diffusion of telomeres in the nucleus of mammalian cells. Physical Review Letters. Jul 3;103(1):018102. 2009. Epub 2009 Jul 2.
28. Dunn KL, He S, Wark L, Delcuve GP, Sun JM, Yu Chen H, Mai S, Davie JR. Increased genomic instability and altered chromosomal protein phosphorylation timing in HRAS-transformed mouse fibroblasts. Genes Chromosomes Cancer 48(5): 397-409. 2009.
29. Knecht H, Sawan B, Lichtensztejn D, Lemieux B, Wellinger RJ, Mai S. The 3D nuclear organization of telomeres marks the transition from Hodgkin to Reed-Sternberg cells. Leukemia 2009 Mar;23(3):565-73. Epub 2008 Nov 27.
30. Gonçalves Dos Santos Silva A, Sarkar R, Harizanova J, Guffei A, Mowat M, Garini Y, Mai S. Centromeres in cell division, evolution, nuclear organization and disease. J Cell Biochem. 2008 Apr 18;104(6):2040-2058.
31. Song H, Mai S, Semegen M. Joining Virtual Reality technology with life science research. Newsletter VRC. Spring 2008.
32. Tragoolpua K, Intasai N, Kasinrerk W, Mai S, Yuan Y, Tayapiwatana C. Generation of functional scFv intrabody to abate the expression of CD147. BMC Biotechnology 8:5. 2008.
33. Guijon FB, Greulich-Bode K, Paraskevas M, Baker P, Mai S. Premalignant cervical lesions are characterized by dihydrofolate reductase gene amplification and c-Myc overexpression: possible biomarkers. J Low Genit Tract Dis. 2007 Oct;11(4):265-72. 
34. Rak M, Del Bigio MR, Mai S, Westaway D, Gough K. Dense-core and diffuse Abeta plaques in TgCRND8 mice studied with synchrotron FTIR Microspectroscopy. Biopolymers. 87(4):207-17. 2007.
35. Gorrini C, Squatrito M, Luise C, Syed N, Perna D, Wark L, Martinato F, Sardella D, Verrecchia A, Bennett S, Confalonieri S, Cesaroni M, Marchesi F, Gasco M, Scanziani E, Capra M, Mai S, Nuciforo P, Crook T, Lough JW, Amati B. Tip60 is a haplo-insufficient tumor suppressor required for an oncogene-induced DNA damage response. Nature 448: 1063-1067. 2007.
36. Guffei A, Lichtensztejn Z, Gonçalves dos Santos Silva A, Louis SF, Caporali A, Mai S. Mouse Robertsonian chromosome formation following c-Myc deregulation. Neoplasia. 9: 578-588. 2007.
37. Zakharenko, LP, Kovalenko, L.V., Mai, S. Fluorescence in situ hybridization analysis of hobo, mdg1 and Dm412 transposable elements reveals genomic instability following the Drosophila melanogaster genome sequencing. Heredity. 2007 Jul 11; [Epub ahead of print]
38. L.P. Zakharenko, L.V. Kovalenko, S. Mai, I.K. Zakharov. Persistent locus-specific instability of yellow2-717 and NotchUc-1 in Drosophila melanogaster coincides with hobo multiplication. Cell and Tissue Research. In press. 2007.
39. Sarkar R, Guffei A, Vermolen BJ, Garini Y, Mai S. Alterations of centromere positions in nuclei of immortalized and malignant mouse lymphocytes. Cytometry Part A. 71(6):386-92. 2007.
40. Caporali A, Wark L, Vermolen BJ, Garini Y, Mai S. Telomeric aggregates and end-to-end chromosomal fusions require myc box II. Oncogene 26(10):1398-1406, 2007. [Epub 2006 Sep 4].
41. Mai S, Imreh S. Non-random genomic instability: A fact, not an illusion. Semin Cancer Biol. 17: 1-4. 2007. [Epub ahead of print: Nov 12, 2006].
42. Kuttler F and Mai S. Formation of non-random extrachromosomal elements during development, differentiation and oncogenesis. Seminars in Cancer Biology 17:56-64, 2007. [Epub ahead of print: 2006 Oct 26].
43. Mai S. The three-dimensional organization of the mammalian nucleus in normal and tumor cells. Atlas of Genetics and Cytogenetics in Hematology and Oncology. November 2005. URL: http://AtlasGeneticsOncology.org/Deep/3DOrganizNuclID20053.html.
44. Intasai N, Mai S, Kasinrerk W, Tayapiwatana C. Binding of multivalent CD147 phage induces apoptosis of U937 cells. Int J Immunol. In press. 2006.
45. Kuttler F and Mai S. c-Myc, genomic instability and disease. In: Volff, J-N. (ed). Genome dynamics. Vol 1: Genome and Disease. Karger Publishers, Wuerzburg, Germany. 171-191. 2006.
46. Cao L, Kim S, Xiao C, Wang R-H, Coumoul X, Wang X, Li WM, Xu XL, De Soto JA, Takai H, Mai S, Elledge SJ, Motoyama N, Deng C-X. ATM-chk2-p53 activation prevents tumorigenesis at an expense of organ homeostasis upon Brca1 deficiency. The EMBO J. 25(10):2167-77. Epub 2006 May 4.
47. Mai S and Garini Y. The significance of telomeric aggregates in the interphase nuclei of tumor cells. Journal Cell Biochem. 97: 904-915. 2006 Jan 11; [Epub ahead of print].
48. Paul JT, Mushinski JF, Henson E, Chuang M, Gibson S, Mai S, Johnston J. Cyclin D expression in chronic lymphocytic leukemia. Leukemia & Lymphoma 46: 1275-85. 2005
49. Mai S and Garini Y. Oncogenic remodeling of the three-dimensional organization of the interphase nucleus: c-Myc induces telomeric aggregates whose formation precedes chromosomal rearrangements. Cell Cycle 4:10, 1327-1331. 2005.
50. Vermolen BJ, Garini Y, Mai S, Mougey V, Fest T, Chuang TCY, Chuang AYC, Wark L, and Young IT. Characterizing the Three-Dimensional Organization of Telomeres. Cytometry. 2005. In press. 
51. Panigrahi S and Mai S. Telomeres, genomic instability, DNA repair and breast cancer. Curr. Med Chem – Anti-Inflammatory & Anti-Allergy Agents 4: 421-428. 2005.
52. Louis S, Gruhne B, Mai S. Towards an understanding of DNA replication in normal and cancer cells: facts and concepts. Review. Progress in Oncogene Research. Chapter I, p. 1-56. Nova Science Publishers, Inc. Hauppauge, NY. 2005.
53. Rak M, Gough K, Del Bigio MR, Mai S, Westaway D. In Situ FTIR Spectromicroscopy of Brain Tissue from a Transgenic Mouse Model of Alzheimer Disease. Vibr Spectrosc 38, 133-141. 2005.
54. Louis SF, Vermolen BJ, Garini Y, Young IT, Guffei A, Zelda Lichtensztejn Z, Fabien Kuttler F, Chuang TCY, Moshir S, Mougey V, Chuang, AYC, Kerr PD, Fest T, Boukamp P, Mai S. c-Myc induces chromosomal rearrangements through telomere and chromosome remodeling in the interphase nucleus. Proc Natl Acad Sci (USA) 102(27): 9613-8. 2005.
55. Fest T, Guffei A, Williams G, Silva S, Mai S. Uncoupling of genomic instability and tumorigenesis in a mouse model of Burkitt’s lymphoma expressing conditional box II-deleted Myc protein. Oncogene 24(18):2944-53. 2005.
56. Kuttler F and Mai S. c-Myc, genomic instability and disease. In: Volff, J-N. (ed). Genome dynamics. Vol 1: Genome and Disease. Karger Publishers, Wuerzburg, Germany. 2005. In press.
57. Mai S, Guffei A, Fest T, Mushinski JF. c-Myc Deregulation Promotes a Complex Network of Genomic Instability. In: Hormonal Carcinogenesis IV (Eds. Li JJ, Li SA, Llombart-Bosch A).Springer-Verlag, New York, pp 87-97. 2005.
58. Ermler S, Krunic D, Knoch TA, Mai S, Greulich-Bode KM, Moshir S and Boukamp P. Cell cycle-dependent 3D distribution of telomeres and TRF2 in HaCaT and HaCaT-myc cells. European J of Cell Biol. Eur J Cell Biol 83: 681-90. 2004. 
59. Louis S, Benedek K, Mowat M, Klein G, Wiener F and Mai S. Elongated mouse chromosomes suitable for enhanced molecular cytogenetics. Cytotechnology 44(3): 143-149. 2004. 
60. Benedek K, Chudoba I, Klein G, Wiener F, Mai S. Rearrangements of the telomeric region of mouse chromosome 11 in Pre-B ABL/MYC cells revealed by mBANDing, spectral karyotyping, and fluoresence in situ hybridization. Chromosome Research 12: 1-9. 2004. 
61. Chuang TCY, Moshir S, Garini Y, Chuang AYC, IT Young, van den Doel R, Mougey V, M Perrin, M Braun, Kerr DP, Fest T, Boukamp P, Mai S. The three-dimensional organization of telomeres in the nucleus of mammalian cells. BMC Biology 2: 12. 2004. 
62. Mai, S and Mushinski JF. c-Myc-dependent genomic instability. J. Environ Path Toxicol Oncol 22: 179-199. 2003.
63. Abrahams BS, Chong ACO, Nisha M, Milette D, Brewster D, Berry ML, Muratkhodjaev F, Mai S, Rajcan-Separovic, Simpsom EM. Metaphase FISHing of transgenic mice recommended: FISH and SKY define BAC-mediated balanced translocation. Genesis 36: 134-41. 2003
64. Smith, G, Dushnicki, L, Smith, S, Wright, JA, Mai, S. c-Myc-induced extrachromosomal elements carry active chromatin. Neoplasia 5: 110-20. 2003. Article and journal cover.
65. Beatty, B, Mai, S., Squire, J. (Eds). FISH: A practical approach. Oxford University Press. 2002.
66. Mai, S. and Wiener, F. Murine FISH. In: FISH: A practical approach. Oxford University Press. 2002.
67. Thierry Fest, T., Mougey, V., Dalstein, V., Hagerty, M.L., Milette, D., Silva, S. and Mai, S. c-MYC overexpression in Ba/F3 cells simultaneously elicits genomic instability and apoptosis. Oncogene 21: 2981-2990. 2002.
68. Mai, S. and Wiener, F. The impact of p53 loss on murine plasmacytoma development. Chromosome Research 10: 239-251. 2002.
69. Kuschak, T.I., Kuschak, B.C., Taylor, C., Wright, J.A., Wiener, F. and Mai, S. c-Myc initiates illegitimate replication of the ribonucleotide reductase R2 gene. Oncogene 21: 909-920. 2002. PMID 11840336
70. Mai, S. and Mushinski, J.F. c-Myc-mediated genomic instability. Critical Reviews in Oncogenesis 21: 31-47. 2001. 
71. Kuschak, T.I., Kuschak, B., Smith, G., Wright, J.A. and Mai, S. Immunoprecipitation of extrachromosomal DNA using anti-histone antibodies. BioTechniques 30(5):1065-1072, 2001. PMID 11355342
72. Hicks, G.G., Singh, N., Nashabi, A., Mai, S., Bozek, G., Klewes, L., White, E.K., 2, Koury, M.J., Oltz, E.M., Van Kaer, L., and Ruley, H.E. Fus deficiency in mice results in defective B lymphocyte development and activation, high levels of chromosomal instability and perinatal death. Nature Genetics. 24: 175-179. 2000. PMID 10655065
73. Wiener, F. and Mai, S. Banding of mouse chromosomes after spectral karyotyping. Technical Tips Online. http://www.biomednet.com/db/tto. T01884. 2000.
74. Kuschak, T.I., Paul, J.T., Wright, J.A., Mushinski, J.F. and Mai, S. FISH on purified extrachromsomal elements. Technical Tips Online. http://www.biomednet.com/db/tto. T01669. 1999.
75. Wiener, F., Kuschak, T.I., Ohno, S. and Mai, S. Deregulated expression of c-Myc in a translocation-negative plasmacytoma on extrachromosomal elements that carry IgH and c-myc genes. Proc. Natl. Acad. Sci (USA). 96 (24):
    13967-13972. 1999.
76. Kuschak, T.I., Taylor, C., McMillan-Ward, E., Israels, S., Henderson, D.W., Mushinski, J.F., Wright, J.A. and Mai, S. The ribonucleotide reductase R2 gene is a non-transcribed target of c-Myc-induced genomic instability. Gene 238: 351-365. 1999. PMID 10570963
77. Mai, S., Hanley-Hyde, J., Rainey, G.J., Kuschak, T.I., Paul, J.T., Littlewood, T.D., Mischak, H., Stevens, L.M., Henderson, D.W., Mushinski, J.F. Chromosomal and extrachromosomal instability of the cyclin D2 gene is induced by Myc overexpression. Neoplasia 1: 241-252. 1999.
78. Taylor, C. and Mai, S. 1998. c-Myc-associated genomic instability of the DHFR locus in vivo. Cancer Detect Prevent. 22: 350-356. 1998. PMID 9674878
79. Fukasawa, K., Wiener, F., Vande Woude, G. F., Mai, S. 1997. Genomic instability and apoptosis are frequent in p53 deficient young mice. Oncogene 15: 1295-1302. PMID 9315097
80. Taylor, C., Jalava, A. and Mai, S. 1997. c-Myc dependent initiation of genomic instability during neoplastic transformation. Current Topics in Microbiol. Immunol. 224: 201-207. PMID 9308243
81. Luecke-Huhle, C., Mai, S., Moll, J. 1997. c-Myc overexpression facilitates radiation-induced DHFR gene amplification. Int. J. Radiat. Res. 71: 167-175.
82. Mai, S., Fluri, M., Siwarski, D., Huppi, K. 1996. Genomic instability in MycER activated Rat1A-MycER cells. Chromosome Research 4: 365-371. PMID 8871825
83. Mai, S., Hanley-Hyde, J., Fluri, M. 1996. c-Myc overexpression associated DHFR gene amplification in hamster, rat, mouse and human cell lines. Oncogene 12: 277-288. PMID 8570205
84. Siegel, J., Fritsche, M., Mai, S., Brandner, G., Hess, R.D. 1995. Enhanced p53 activity and accumulation in response to DNA damage. Oncogene 11: 1363-1370. PMID 7478558
85. Mai, S., Hanley-Hyde, J., Coleman, A., Siwarski, D., Huppi, K. 1995. Amplified extrachromosomal elements containing c-Myc and Pvt 1 in a mouse plasmacytoma. Genome 38:780-785. PMID 7672608

PubMed Listed Publications

Visiting scientists and students, 2002.

Dr. Chatchai Tayapiwatana, University of Chiang Mai, Chiang Mai, Thailand.
Virginie Mougey, University of Besançon, Besançon, France.
Dr. Thierry Fest, Univeristy of Besançon, Besançon ,France.
Dr. Francis Wiener, Karolinska Institute, Stockholm, Sweden.
Dr. Karin Greulich-Bode, German Cancer Research Center, Heidelberg, Germany.
Sharareh Moshir, German Cancer Research Center, Heidelberg, Germany.

Visiting scientists and students, 2003.

Sibylle Ermler, German Cancer Research Center, Heidelberg, Germany
Dr. Thierry Fest, Univeristy of Besançon, Besançon ,France.
Sharareh Moshir, German Cancer Research Center, Heidelberg, Germany
Mathilde Perrin, University of Besancon, Besacon France
Dr. Francis Wiener, Karolinska Institute, Stockholm Sweden
Colleen Fordyce, University of California San Francisco, USA
Hal Berman, University of California San Francisco, USA
Caroline Miller, University of California San Francisco, USA
Virginie Mougey, University of Besançon, France
Nutjeera Intasai, University of Chiang Mai, Thailand

Visiting scientists and students, 2004:

Iris Gehrke, University of Cologne, Germany
Nutjeera Intasai, University of Chiang Mai, Thailand
Andrea Caporali, University of Parma, Italy
Dr. Thierry Fest, University of Rennes, France
Bettina Gruhne, University of Tuebingen, Germany

Visiting scientists and students, 2005:

Amanda Gonçalves Da Silva, Unversity of Sao Paulo, Brazil
Andrea Caporali, University of Parma, Italy
Iris Gehrke, University of Cologne, Germany
Nutjeera Intasai, University of Chiang Mai, Thailand
Dr. Eva Darai, MTC, Karolinska Institute, Stockholm, Sweden
Dr. Maria Kost-Alimova, MTC, Karolinska Institute, Stockholm, Sweden
Johannes Kohlschuetter, University of Freiburg, Germany.
Dr. Liudmila P. Zhakharenko, Institute of Cytology and Genetics, Siberian Department of the Russian Academy of Science, Novosibirsk, Russia

Visiting scientists and students, 2006:

Dr. Anna Szeles, MTC, Karolinska Institute, Stockholm Sweden
Fabio Morato de Oliveira, University of Sao Paulo, Brazil
Dr. Hans Knecht, University of Sherbrooke, Quebec
Dr. Qing Li, South Bend Medical Foundation, South Bend , IN, USA
Amanda Gonçalves da Silva, University of Sao Paulo, Brazil
Filip Misev, University of Basel, Switzerland.

Visiting scientists and students, 2007:

Dr. Yuval Garini, Bar Ilan University, Israel
Dr. Hans Knecht, University of Sherbrooke, Quebec
Dr. Nutjeera Intasai, University of Chiang Mai, Thailand
Dr. Chatchai Tayapiwatana, University of Chiang Mai, Thailand
Amanda Gonçalves dos Santos Silva, University of Sao Paulo, Brazil
Emilia Wiechec, University of Aarhus, Denmark

Visiting scientists 2008:

Dr. Birthe Fahrenkrog, University of Basel, Switzerland
Dr. Ignacio Gonzalez-Suarez, Washington University School of Medicine, St. Louis, USA
Macoura Gadji, University of Sherbrooke, QC, Canada
Amanda Gonçalves dos Santos Silva, Universidade Federal de São Paulo, SP, Brazil
Hans Knecht, University of Sherbrooke, QC, Canada
Dr. Irena Bronshtein, Bar Ilan University, Israel
Dr. Johannes Von Vopelius-Feldt, Technical University of Munich, Germany
Dr. Nutjeera Intasai, Chiang Mai University, Thailand
David Novak, McGill University, QC, Canada
Dr. Miri Blank, Tel Hashomer, Israel
Lorri Martin, Cross Cancer Centre, Edmonton, AB, Canada
Ann-Kristin Schmälter, Helmholtz-Gymnasium Bonn-Duisdorf, Germany
Dr. Tamara Lushnikova, Nashville TN, USA
Dr. Kirk McManus, Vancouver, BC, Canada

Visiting scientists 2009:

Dr. Hans Knecht, University of Sherbrooke (QC)
Macoura Gadji, University of Sherbrooke (QC)
Oumar Samassekou, Univeristy of Sherbrooke (QC)
Dr. Birthe Fahrenkrog, University of Basel, Swizterland
Dr.Ignatio Gonzalez-Suarez, Washington University School of Medicine, St Louis, USA
Amanda Gonçalves dos Santos Silva, Universidade Federal de São Paulo, SP, Brazil
Ann-Kristin Schmälter, University of Munich, Medical School, Germany
Christiaan Righolt, University of Technology, Delft, The Netherlands

Visiting scientists 2010:

Dr. Hans Knecht, University of Sherbrooke (QC)

Visiting scientists 2011:

Dr. Hans Knecht, CHUS, U of Sherbrooke, QC, Canada
Dr. Catalena Birek, U of M, Department of Oral Biology, Winnipeg, MB, Canada
Julius Awe Adebayo, U of Skovde, Sweden
Ann-Kristin Schmaelter, TU Munich, Germany

Lab Members: