October 2003 Workshop
Fluorescent immunohistochemistry with anti-histone H3P (Upstate
Cell Signaling) on Pro-B lymphocytes; image acquired using DAPI-FITC
Fluorecent in situ hybridization using mouse chromosome 11 paint
(Cy3) from CedarLane/Cambio. The cells used are mouse pro-B cells
with near tetraploid chromosome constitution.
1. The participants of the workshop “Principles
of Microscopy and Imaging”, October 2003.
2. Lecture by Robert Parsons (Zeiss).
3. Training on live cell imaging with Hal Arthurs
4. Bench work – preparation for fluorescent
in situ hybridization (FISH) and imaging.
5. Lecture on FISH and related technologies by Dr.
Ulrich Klingbeil (MetaSystems Group Inc.).
6. The group listens to lectures in the Dr. Arnold
Greenberg Lecture Hall at the Manitoba Institute of Cell Biology.
7. The training also offers career orientation to
high school students. Sourabh Maiti, a Senior III student at Vincent
Massey Collegiate acquires images.
8. At the end of each workshop there is a written
test; the participants are writing their final exam.
9. Workshop dinner at the end of the workshop.
"How does it feel to be a biologist?"
"Bennie, the bee..."
Career goal: astronaut.