| MBIO
3470 Teaching Assistants:
Taylor Mullineux - 401 Buller (scheduled lab time)
Mohamed Abdel-Fattah, Robin Imperial, Aneil Moya Torres (during the week
supervisors)
All lab reports must have a completed
Honesty Declaration attached: Group
Lab Assignment or Individual
Lab Assignment. Available as pdf.
Lab 1 streak plate report marks
MBIO 3470 LAB INFORMATION
Lab starts Wednesday, January 13, 2010 in room
302 Buller. Lab manuals are handed out in class the first week of lectures.
All essential lab related information is given in the lab manual.
Students divided alphabetically by last
name into two sections:
Section 1 (L-Z) starts at 2:30 pm
Section 2 (A-K) starts at 3:30 pm the first week (see general instructions
in your lab manual for other start times)
If you wish to switch sections, you must find a student
in the other section willing to change sections, then confirm with me.
Section student division is subject to change if
enrolment distribution of last names change prior to start of lab.
The first lab starts with a SOP and Safety quiz (10 min), come
prepared. SOP and laboratory biosafety guide information
Come prepared the first lab ready to comply with all standard
operations procedures.
Reminders:
You need a separate lab coat for MBIO 3470 that stays in the lab. You
must bring your lab coat the first lab and a bag to hold your lab coat.
No outer clothing or back packs allowed in the lab, only lab manual, notebook
and pens. Small lockers available in the hall outside the lab. Long hair
must be tied back. No open toed shoes.
Lab Safety:
UM
Environmental Health and Safety Office (EHSO) -
chemical and biological safety
Basic
Lab Safety and WHMIS (PowerPoint presentation)
Biohazard
MSDS
Read lab report presentation section in general instructions at the beginning of your lab manual before doing your lab report. Lab reports must typed. This includes calculations. 10% of the mark subtracted for portion of report not typed.
LAB REPORT Data tables/formats
(Word format):
lab 1 Identification Test results
and interpretation, lab
2, lab 3, lab
4 Isolate Data Sheet,
lab 4 ATB G-antibiotic analysis table,
lab 5, lab 6. Save
Word document and open in Word before recording requested information.
For lab 3, 4 and 6 word format report tables (APIPLUS table has been removed
as no longer required), just include APIWEB printout.
LAB REPORT Data table (Excel format):
lab 1 Numerical data
sheet. Save spreadsheet and open in Excel before recording requested
information. The spread sheet has been set to automatically give you the
total numerical value for each bacterium.
Lab 4 Enterobacteriaceae: Isolation Identification
and Antibiotic testing information
Cephalosporins
Microbial Systematics Lab Manual
(numerous tables/diagrams are missing as compared to lab manual
handed out in class.
Lab 1 Table 1. Biochemical
Characteristics of selected members of Enterobacteriaceae (pdf)
Lab 1 Table 2. Biochemical
Characteristics Data Sheet (pdf)
Lab 3 blank API sheets
Lab 3 API Identification Table: page
1 and page 2
Lab 6 blank API 20NE
data sheets
Gram Stain addendum - the company has notified us that the safranin counter
stain contains gram positive coccoid artifacts. The coccoids are artifacts
of the stain, not culture contamination.
LAB 5 Streptococcaceae
Streptococcus hemolysis data
Scan of Streptococcus pyogenes, Streptococcus agalactiae, and
Streptococcus faecalis growing on sheep blood agar plates. Incubated
at 37C for two days.
Spanu,
T, Sanguinetti, M, Ciccaglione, D, D’Inzeo, T, Romano, L, Leone,
F, Fadda, G. 2003. Use of the VITEK 2 System for rapid identification
of clinical isolates of Staphylococci and bloodstream infections. J. Clin.
Micro. 41: 4259-4263
Wellinghausen,
N, Wirths, B, Essig, A, Wassill, L. 2004. Evaluation of the Hyplex
Bloodstream Multiplex PCR-enzyme-linked immunosorbent assay system for
direct identification of Gram-postive cocci and Gram-negative bacilli
from positive blood cultures. J. Clin. Micro. 42: 3147-3152.
Biosafety
MSDS (infectious
agents)
Faculty
of Science Suggested Minimum Penalties for Common Acts of Academic Dishonesty
Faculty
of Science Academic Dishonesty website
Frequently information is available as
a pdf file requiring Adobe Acrobat Reader. Adobe abcrobat reader is available
at UM Software ExpressUM
Software Express.
Lab Skill Sheet-
available for students to use for job applications, just check off the
highlighted boxes for the lab course(s) you have taken.
pdf or excel (prior to Fall 2007)
pdf or
excel (starting Fall 2007)
email le_cameron@umanitoba.ca
Emails: subject must contain course number and subject,
eg. 3470 lab 1 report. If no subject given, email is deleted. Emails replies
occur only during working hours. Email must include student name.
phone 474-6542
Department of Microbiology
Buller Bldg
Winnipeg, MB
R3T 2N2
University
of Manitoba calender 2008 -2009
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