Research Institute in Oncology and Hematology


Contact the GCCRD:

Ludger Klewes
ON6026-675 McDermot Ave
Winnipeg, Manitoba, Canada R3E 0V9
Phone: 204-787-2170 or 204-787-2760


Contact RIOH:

Research Institute in Oncology and Hematology
675 McDermot Ave
Winnipeg, Manitoba, Canada R3E 0V9
Phone: 204-787-2137
Fax: 204-787-2190
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The Genomic Centre for Cancer Research and Diagnosis

Equipment

The GCCRD has been designed as a high-technology facility for digital imaging and analysis. The activities havebeen divided into two major areas, basic research and technical services, that are envisioned to promote a better interaction between different fields of research.

The basic research component of the GCCRD is developed on collaboration with scientific groups from Canada, USA, Asia and Europe. Technical services, on the other hand, are offered on a fee basis, and these are mainly forcused on fluorescent in situ hybridization, immunohistochemistry, and microdissection of biological material. The Centre does not carry out clinical diagnostics, but collborations with clinical genetics laboratories are strongly encouraged.

Description of Workstations
ASI Scanner-1
  1. Olympus BX61 microscope with 8-slide stage
  2. ASI ScanView camera
  3. Olympus BX-UCB light source
  4. Microsoft Windows X6 Professional Version 2002 Service Pack 3; Intel "Xeon" CPU W3530 @ 2.80 GHz
  5. Case Data Manager Version 6.0.016 Copyright 2003-2009
Applications: Scanning, tissue arrays, immunofluorescence.
ASI Scanner-2
  1. Zeiss AxioImager Z2 microscope with 8-stage slide
  2. ASI ScanView camera
  3. Zeiss X-Cite light source
  4. Windows 7 Ultimate© 2009 Microsoft Corporation Intel "Xeon" CPU X5650 2.67GHz (2 processors)
  5. GenASIs 7.2.5
Applications: Scanning, tissue arrays, immunofluorescence.
Deconvolution-1 Station
  1. Zeiss AxioImager Z2 microscope
  2. Zeiss AxioCam HRm
  3. Windows 7 Ultimate© 2009 Microsoft Corporation Intel "Xeon" CPU X5650 2.67GHz (2 processors)
  4. AxioVision Release 4.8.2 (2006-2010) Carl Zeiss MicroImaging GmbH
Applications: 2D- and 3D- FISH and immunofluorescence
Deconvolution-2 Station
  1. Zeiss AxioImager Z2 microscope
  2. Zeiss AxioCam MRm
  3. Windows 7 Ultimate© 2009 Microsoft Corporation Intel "Xeon" CPU X5650 2.67GHz (2 processors)
  4. AxioVision Release 4.8.2 (2006-2010) Carl Zeiss MicroImaging GmbH
Applications: 2D- and 3D- FISH and immunofluorescence
Laser Micro-Dissection Station
  1. Zeiss Axioskop 2 microscope
  2. Zeiss MC 200 CHIP camera
Applications: Laser microdissection of chromosomes, extra-chromosomal DNA, and single cells.
The system is also coupled with a Sutter glass puller for custom-made glass micropipettes.
Live Cell Station
  1. Zeiss Axiovert 200M
  2. Zeiss AxioCam MRm and AxioCam HSm
  3. Xeon CPU 2.80 GHz, Microsoft Windows XP Professional Version 2002 5.1.2600 Service Pack 2 Build 2600
  4. AxioVision Release 4.6.3 (04-2007)
Applications:Fluorescence and brightfield imaging of live cells and quantification of fluorescent signals.
System is equipped to provide a humidified, temperature-controlled, and 5% C02 environment.
Metasystems Station
  1. Zeiss Axioplan 2 microscope
  2. MetaSystems camera
  3. Pentium 4 CPU 2.80 GHz, Microsoft Windows XP Professional Version 2002 Service Pack 2
  4. MetaSystems Isis/Ikaros version 5.4.9 (4/8/2011)
Applications:Fluorescence in situ hybridization (FISH), multicolour-FISH (M-FISH), quantitative-FISH (Q-FISH), karyotyping (fluorescent and brightfield), and comparative genomic hybridization (CGH).
Pathology Station
  1. Zeiss Axioskop2 microscope
  2. Zeiss AxioCam ICc3 colour camera
  3. Pentium 4 CPU 3.00 Ghz, Microsoft Windows XP Professional Version 2002 Service Pack 3
  4. AxioVision Release 4.8 (06-2009), Carl Zeiss Imaging Solutions GmbH
Applications:Brightfield microscopy of chromosomes, cells, and tissues.
Scanning Metasystems Station
  1. Zeiss Axioplan 2 microscope
  2. Zeiss AxioCam MRm
  3. Intel Core i7 CPU 860 @ 2.80GHz, Microsoft Windows XP x32 5.1Service Pack 3
  4. MetaSystems Isis mFISH/mBAND, CGH, Telomere Measurement System, version 5.4.9 (4/18/2011), Metafer Slide Scanning System version 3.8 (2010 MetaSystems GmbH)
Applications:Fluorescence in situ hybridization (FISH), multicolour-FISH (M-FISH), quantitative-FISH (Q-FISH), karyotyping (fluorescent and brightfield); multiple slide platform for metaphase, cells, and FISH signal detection.
Spectral Karyotyping (SKY) Station
  1. Zeiss Axioplan 2 microscope
  2. ASI SpectraCubeTM Model COOL-1300QS
  3. Intel "Pentium" 4 CPU 3.00 GHz, Microsoft Windows XP Professional Version 2002 Service Pack 3
  4. Case Data Manager Version 6.0.0.16 Copyright 2003-2009 (includes the software Spectral Imaging, SkyView, FishView, and BandView)
Applications:Spectral karyotyping (SKY) of mouse and human chromosomes, FISH, CGH, CGH array, spectral FISH, G-banding, karyotyping.
Super Resolution Imaging Station
  1. Zeiss AxioObserver.Z1
  2. Zeiss ELYRA PS.1 module
  3. Zeiss camera Andor iXon 885
  4. LSM 710 34-channel light source
  5. SR SIM+PALM
  6. Windows 7 Ultimate Copyright 2009 Microsoft Corporation Intel "Xeon" CPU X5650 2.67 GHz (2 processors)
  7. Carl Zeiss Zen 2010 D (64-bit) LSM 7 Elyra Release Version 7.0 Carl Zeiss MicroImaging GmbH 1997-2001
Applications: 2- and 3D high-resolution (20 nm) fluorescence imaging, laser scanning microscopy, FRET, FRAP, RICS, PALM, SIM.
Z-1
  1. Zeiss AxioImager Z1 microscope
  2. Zeiss Axio Cam HRm
  3. Zeiss Apotome system
  4. Windows 7 Ultimate© 2009 Microsoft Corporation Intel "Xeon" CPU X5650 2.67GHz (2 processors)
  5. AxioVision Release 4.8.1 (11-2009) Carl Zeiss Imaging Solutions GmbH
Applications: 2D and 3D fluorescence microscopy, FISH, Q-FISH, immunofluorescence.
Data Analysis Station
  1. Intel "Xeon" CLU E5506 2.13 GHz, Windows VistaTM Ultimate, Copyright 2007 Service Pack 2
  2. AxioVision Release 4.8.2 (06-2010), Carl Zeiss MicroImaging GmbH
Applications: Image processing ,deconvolution, analysis
MMI cellcut plus
  1. Olympus IX 71 inverted microscope
  2. HP Z210 system Intel Core 7 3.1MHz
Applications: UV laser & adhesive caps allow for isolation of pure cells or tissue sections from slide
Server connects all computers in the GCCRD
  1. Windows2K3 and Mac servers recently upgraded to 30TB of storage space with redundant backup storage to come.
Applications: UV laser & adhesive caps allow for isolation of pure cells or tissue sections from slide



RIOH gratefully acknowledges the generous support of



CCMB_Logo RIOH is a joint institute of CancerCare Manitoba and University of Manitoba UofM_Logo