Research Institute in Oncology and Hematology

Sabine Mai

Genomic Instability

Professor Physiology, Biochemistry & Medical Genetics, Human Anatomy and Cell Science
Director of The Genomic Centre for Cancer Research and Diagnosis (GCCRD)

B.Sc. in Biology, Univ. of Cologne, Germany, 1981
M.Sc. in Biology, French & Pedogogics, Univ. of Cologne, Germany, 1985
Ph. D. in Molecular Biology, Univ of Karlsruhe, Germany, 1991

6046-675 McDermot Ave
Winnipeg, Mb R3E 0V9
(204) 787-2135
Dr Sabine Mai PhD

Publications since 2010

PubMed Listed Publications
  1. Distinct and shared three-dimensional chromosome organization patterns in lymohocytes, monocloncal gammopathy of undetermined significance and multiple myeloma. Sathitruangsak C, Righolt CH, Klewes L, Chang DT, Kotb R, Mai S. Int J Cancer. 2016. Accepted for publication.
  2. XPO1 Inhibition Preferentially Disrupts the 3D Nuclear Organization of Telomeres in Tumor Cells. Taylor-Kashton C, Lichtensztejn D, Baloglu E, Senapedis W, Shacham S, Kauffman MG, Kotb R, Mai S. J Cell Physiol. 2016 Dec;231(12):2711-9. doi: 10.1002/jcp.25378. Epub 2016 Apr 8.
  3. Circulating Tumor Cells (CTC) and Cell-Free DNA (cfDNA) Workshop 2016: Scientific Opportunities and Logistics for Cancer Clinical Trial Incorporation. Lowes LE, Bratman SV, Dittamore R, Done S, Kelley SO, Mai S, Morin RD, Wyatt AW, Allan AL. Int J Mol Sci. 2016 Sep 8;17(9). pii: E1505. doi: 10.3390/ijms17091505.
  4. FGFR3 preferentially colocalizes with IGH in the interphase nucleus of multiple myeloma patient B-cells when FGFR3 is located outside of CT4. Martin LD, Harizanova J, Mai S, Belch AR, Pilarski LM. Genes Chromosomes Cancer. 2016 Aug 11. doi: 10.1002/gcc.22394. [Epub ahead of print]
  5. Identification of Neuroblastoma Subgroups Based on Three-Dimensional Telomere Organization. Kuzyk A, Gartner J, Mai S. Transl Oncol. 2016 Aug;9(4):348-56. doi: 10.1016/j.tranon.2016.07.001.
  6. DNA Superresolution Structure of Reed-Sternberg Cells Differs Between Long-Lasting Remission Versus Relapsing Hodgkin's Lymphoma Patients. Righolt CH, Knecht H, Mai S. J Cell Biochem. 2016 Jul;117(7):1633-7. doi: 10.1002/jcb.25456. Epub 2015 Dec 31.
  7. Three-dimensional telomere architecture of esophageal squamous cell carcinoma: comparison of tumor and normal epithelial cells. Sunpaweravong S, Sunpaweravong P, Sathitruangsak C, Mai S. Dis Esophagus. 2016 May;29(4):307-13. doi: 10.1111/dote.12317. Epub 2015 Jan 27.
  8. An intact putative mouse telomerase essential N-terminal domain is necessary for proper telomere maintenance. Rousseau P, Khondaker S, Zhu S, Lauzon C, Mai S, Autexier C. Biol Cell. 2016 Apr;108(4):96-112. doi: 10.1111/boc.201500089. Epub 2016 Feb 18.
  9. Assessment of the clinical relevance of 17q25.3 copy number and three-dimensional telomere organization in non-small lung cancer patients. Sunpaweravong P, Thu KL, Lam WL, Mai S. J Cancer Res Clin Oncol. 2016 Apr;142(4):749-56. doi: 10.1007/s00432-015-2080-5. Epub 2015 Nov 30.
  10. Editorial: Towards New Approaches in Alzheimer's Research and Alzheimer's Disease. Mai S. Curr Alzheimer Res. 2016;13(7):728-9. No abstract available.
  11. DNA methylation screening of primary prostate tumors identifies SRD5A2 and CYP11A1 as candidate markers for assessing risk of biochemical recurrence. Horning AM, Awe JA, Wang CM, Liu J, Lai Z, Wang VY, Jadhav RR, Louie AD, Lin CL, Kroczak T, Chen Y, Jin VX, Abboud-Werner SL, Leach RJ, Hernandez J, Thompson IM, Saranchuk J, Drachenberg D, Chen CL, Mai S, Huang TH. Prostate. 2015 Nov;75(15):1790-801. doi: 10.1002/pros.23052. Epub 2015 Sep 1.
  12. MYCN overexpression is associated with unbalanced copy number gain, altered nuclear location, and overexpression of chromosome arm 17q genes in neuroblastoma tumors and cell lines. Kuzyk A, Booth S, Righolt C, Mathur S, Gartner J, Mai S. Genes Chromosomes Cancer. 2015 Oct;54(10):616-28. doi: 10.1002/gcc.22273. Epub 2015 Jul 14.
  13. Changes in Nuclear Orientation Patterns of Chromosome 11 during Mouse Plasmacytoma Development. Schmälter AK, Righolt CH, Kuzyk A, Mai S. Transl Oncol. 2015 Oct;8(5):417-23. doi: 10.1016/j.tranon.2015.09.001.
  14. Loss of lamin A function increases chromatin dynamics in the nuclear interior. Bronshtein I, Kepten E, Kanter I, Berezin S, Lindner M, Redwood AB, Mai S, Gonzalo S, Foisner R, Shav-Tal Y, Garini Y. Nat Commun. 2015 Aug 24;6:8044. doi: 10.1038/ncomms9044.
  15. MYCN overexpression is associated with unbalanced copy number gain, altered nuclear location and overexpression of chromosome 17q genes in neuroblastoma tumors and cell lines. 2015 Kuzyk A, Booth S, Righolt C, Mathur S, Gartner J, Mai S. Genes Chromosomes Cancer 2015 Oct;54(10):616-28. doi: 10.1002/gcc.22273. Epub 2015 Jul 14.
  16. DNA methylation screening of primary prostate tumors identifies SRD5A2 and CYP11A1 as candidate markers for assessing risk of biochemical recurrence. Horning AM, Awe JA, Wang CM, Liu J, Lai Z, Wang VY, Jadhav RR, Louie AD, Lin CL, Kroczak T, Chen Y, Jin VX, Abboud-Werner SL, Leach RJ, Hernandez J, Thompson IM, Saranchuk J, Drachenberg D, Chen CL, Mai S, Huang TH. Prostate. 2015 Sep 1. doi: 10.1002/pros.23052. [Epub ahead of print]
  17. Measuring murine chromosome orientation in interphase nuclei. Righolt CH, Schmälter A-K, Kuzyk A, Young IT, van Vliet LJ, Mai S. Cytometry A. 2015 Apr 17. doi: 10.1002/cyto.a.22674. [Epub ahead of print]
  18. Three-dimensional telomere architecture of esophageal squamous cell carcinoma: comparison of tumor and normal epithelial cells. Sunpaweravong S, Sunpaweravong P, Sathitruangsak C, Mai S. Dis Esophagus. 2015 Jan 27. doi: 10.1111/dote.12317. [Epub ahead of print]
  19. LMP1 mediates multinuclearity through downregulation of shelterin proteins and formation of telomeric aggregates. Lajoie V, Lemieux B, Sawan B, Lichtensztejn D, Lichtensztejn Z, Wellinger R, Mai S, Knecht H. Blood. 125 (13): 2101-2110. 2015 Jan 7. pii: blood-2014-08-594176. [Epub ahead of print], cover and highlight.
  20. Quantitative Superresolution Microscopy Reveals Differences in Nuclear DNA Organization of Multiple Myeloma and Monoclonal Gammopathy of Undetermined Significance. Sathitruangsak C, Righolt CH, Klewes L, Tammur P, Ilus T, Tamm A, Punab M, Olujohungbe A, Mai S. J Cell Biochem 2014 Dec 10. doi: 10.1002/jcb.25030. [Epub ahead of print]
  21. Different TP53 mutations are associated with specific chromosomal rearrangements, telomere length changes, and remodeling of the nuclear architecture of telomeres. Samassekou O, Bastien N, Lichtensztejn D, Yan J, Mai S, Drouin R. Genes Chromosomes Cancer. 2014 Nov;53(11):934-50. doi: 10.1002/gcc.22205. Epub 2014 Jul 25.
  22. Distinct nuclear orientation patterns for mouse chromosome 11 in normal B lymphocytes. 2014 Schmälter AK, Kuzyk A, Neusser M, Steinlein OS, Müller S, Mai S.BMC Cell Biology. 2014 Jun 12;15(1):22. [Epub ahead of print]
  23. Three-dimensional structured illumination microscopy using Lukosz bound apodization reduces pixel negativity at no resolution cost. Righolt CH, Mai S, van Vliet LJ, Stallinga S. Opt Express. 2014 May 5;22(9):11215-27. doi: 10.1364/OE.22.011215.
  24. Rapid separation of mononuclear hodgkin from multinuclear reed-sternberg cells. Kongruttanachok N, Cayre YE, Knecht H, Mai S.Lab Hematol. 2014 Mar 1;20(1):2-6. doi:10.1532/LH96.12023.
  25. Differences in nuclear DNA organization between lymphocytes, Hodgkin and Reed-Sternberg cells revealed by structured illumination. Righolt CH, Guffei A, Knecht H, Young IT, Stallinga S, van Vliet L, Mai S. J Cell Biochem. 2014 Mar 4. doi: 10.1002/jcb.24800. [Epub ahead of print]
  26. Pristane plus v-abl/myc induced lymphoma and plasmacytoma development in STS/A mice. 2014. Sakulratchata R, Klein G, Demant P, Mai S. Naresuan University.
  27. 3D nuclear telomere organization in multiple myeloma. Klewes L, Vallente R, Dupas E, Brand C, Grün D, Guffei A, Sathitruangsak C, Awe JA, Kuzyk A, Lichtensztejn D, Tammur P, Ilus T, Tamm A, Rubinger M, Olujohungbe A, Mai S. Translational Oncology 6(6): 749-756. 2013
  28. Image filtering in structured illumination microscopy using the Lukosz bound. Righolt CH, Slotman JA, Young TI, Mai S, van Vliet LJ, Stallinga S. Optics Express 21(21):24431-51. 2013.
  29. A new der(1;7)(q10;p10) leading to a singular 1p loss, in a case of glioblastoma with oligodendroglioma component. Gadji M, Crous-Tsanaclis A-M, Mathieu D, Mai S, Fortin D, Regen D. Neuropathology. 2013. In press.
  30. Three-dimensional quantitative imaging of telomeres in buccal cells identifies mild, moderate and severe Alzheimer patients. Mathur S, Glogowska A, McAvoy E, Righolt C, Rutherford C, Willing C, Banik U, Ruthirakuban M, Mai S*, Garcia A*. J of Alzheimer's Disease. 2013, In press. *co-corresponding authors.
  31. Three-dimensional nuclear telomere architecture changes during endometrial cancer development. Danescu A, Herrero Gonzalez S, Di Cristofano A, Mai S and Hombach-Klonisch S. Genes Chromosomes Cancer 2013. Apr 30. doi: 10.1002/gcc.22067. [Epub ahead of print]
  32. 3D nuclear organization and genome stability in cancer. Keynote presentation. Mai S. BMC Proceedings 2013, 7(Suppl 2):K17.
  33. Genomic Instability: The Driving Force behind Refractory/Relapsing Hodgkin's Lymphoma. Knecht H, Righolt C, Mai S. Cancers 5(2), 714-725. 2013. doi:10.3390/cancers5020714
  34. Three dimensional (3D) telomere dynamics in follicular thyroid cancer. Wark L, Danescu A, Natarajan S, Zhu X, Cheng S-y, Hombach-Klonisch S, Mai S*, Klonisch T*. Thyroid. In press. *co-corresponding authors
  35. Three dimensional nuclear imaging and cytogenetic characterization of cell lines heterozygous for mutations in the PALB2 hereditary breast cancer predisposition gene. Wark L, Novak D, Sabbaghian N, Amrein L, Jangamreddy JR, Cheang M, Pouchet C, Aloyz R, Foulkes WD, Mai S, Tischkowitz M. Genes Chromosomes Cancer 2013 Jan 23. doi: 10.1002/gcc.22045. [Epub ahead of print]
  36. Nuclear remodeling of telomeres in chronic myeloid leukemia. Samassekou O, Hebert, J, Mai S, Yan J. Genes Chromosomes Cancer 2013. Jan 23. doi: 10.1002/gcc.22046. [Epub ahead of print]
  37. Differential nuclear organization of translocation-prone genes in nonmalignant B cells from patients with t(14;16) as compared with t(4;14) or t(11;14) myeloma. Martin LD, Harizanova J, Righolt CH, Zhu G, Mai S, Belch AR, Pilarski LM. Genes Chromosomes Cancer. 2013 Mar 5. doi: 10.1002/gcc.22049. [Epub ahead of print]
  38. Three-Dimensional Telomeric Analysis of Isolated Circulating Tumor Cells (CTCs) Defines CTC Subpopulations. Adebayo Awe J, Xu MC, Wechsler J, Benali-Furet N, Cayre YE, Saranchuk J, Drachenberg D, Mai S. Transl Oncol. 2013 Feb;6(1):51-65. Epub 2013 Feb 1.
  39. Mining gene expression signature for the detection of pre-malignant melanocytes and early melanomas with risk for metastasis. de Souza CF, Xander P, Monteiro AC, Silva AG, da Silva DC, Mai S, Bernardo V, Lopes JD, Jasiulionis MG. PLoS One. 2012;7(9):e44800. Epub 2012 Sep 11.
  40. Shattered and stitched chromosomes-chromothripsis and chromoanasynthesis-manifestations of a new chromosome crisis? Righolt C, Mai S. Genes Chromosomes Cancer. 2012 Jul 18. doi: 10.1002/gcc.21981
  41. 3D telomere signatures of Hodgkin- and Reed-Sternberg cells at diagnosis indicate refractory/relapsing Hodgkin's lymphoma. Knecht H, Kongruttanachok N, Sawan B, Brossard J, Prevost S, Turcotte E, Lichtensztejn Z, Lichtensztejn D, Mai, S. Translational Oncology. Aug;5(4):269-77. 2012. Epub 2012 Aug 1.
  42. Selected telomere length changes and aberrant three-dimensional nuclear telomere organization during fast-onset mouse plasmacytomas. Kuzyk A, Mai S. Neoplasia. 2012 Apr;14(4):344-51. PubMed PMID: 22577349; PubMed Central PMCID: PMC3349260.
  43. Profiling Three-Dimensional Nuclear Telomeric Architecture of Myelodysplastic Syndromes and Acute Myeloid Leukemia Defines Patient Subgroups. Gadji M, Adebayo Awe J, Rodrigues P, Kumar R, Houston DS, Klewes L, Dieye TN, Rego EM, Passetto RF, de Oliveira FM, Mai S. Clin Cancer Res. 2012 Jun 15;18(12):3293-3304. Epub 2012 Apr 26. PubMed PMID: 22539801.
  44. Loss of HLTF function promotes intestinal carcinogenesis. Sandhu S, Wu X, Nabi Z, Rastegar M, Kung S, Mai S, Ding H. Mol Cancer. 2012 Mar 27;11:18. PubMed PMID: 22452792; PubMed Central PMCID: PMC3337324.
  45. Differential positioning and close spatial proximity of translocation-prone genes in nonmalignant B-cells from multiple myeloma patients. Martin LD, Harizanova J, Zhu G, Righolt CH, Belch AR, Mai S, Pilarski LM. Genes Chromosomes Cancer. 2012 Aug;51(8):727-42. doi: 10.1002/gcc.21959. Epub 2012 Apr 9. PubMed PMID: 22489023.
  46. Recurrent trisomy and Robertsonian translocation of chromosome 14 in murine iPS cell lines. Chen Q, Shi X, Rudolph C, Yu Y, Zhang D, Mai S, Wang G, Schlegelberger B, Shi Q. Chromosome Research 2011. In press.
  47. Nuclear remodeling as a mechanism for genomic instability in cancer. Gadji M, Vallente R, Klewes L, Righolt C, Wark L, Kongruttanachok N, Knecht H, Mai S. Adv Cancer Res. 2011; 112:77-126. Article and cover.
  48. Inversion and deletion of 16q22 defined by array CGH, FISH, and RT-PCR in a patient with AML. Dawson AJ, Bal S, McTavish B, Tomiuk M, Schroedter I, Arshad N. Ahsanuddin AN, Seftel MD, Vallente R, Mai S, Cotter PD, Hovanes K, Gorre M, Gunn SR. Cancer Genetics 204 (2011) 344e347.
  49. Translocation frequencies and chromosomal proximity for selected mouse chromosomes in primary mouse B lymphocytes. Righolt C, Wiener F, Taylor-Kashton C, Harizanova J, Vermolen B, Garini Y, Young IT, Mai S. Cytometry Part A. 79A: 276-283, 2011.
  50. Cyclin E amplification/overexpression is a mechanism of trastuzumab resistance in HER2 positive breast cancer patients. Scaltriti M, Eichhorn PJA, Cortes J, Prudkin L, Aura C, Jimenez J, Chandarlapaty S, Serra V, Prat A, Ibrahim YH, Guzman M, Gili M, Rodriguez O, Rodriguez S, Perez J, Green SR, Mai S, Rosen N, Hudis C and Baselga J. Proc Natl Acad Sci (USA). 2011. Feb 14.
  51. Novel automated three-dimensional (3D) genome scanning based on the nuclear architecture of telomeres. Klewes L, Hobsch C, Katzir, N, Rourke D, Garini Y, Mai S. Cytometry Part A. 2011 Feb;79(2):159-66. doi: 10.1002/cyto.a.21012. Epub 2010 Dec 30.
  52. 3D imaging of telomeres and nuclear architecture : an emerging tool of 3D nano-morphology based diagnosis. Knecht H, Mai S. J Cell Physiol. 2011 Apr;226(4):859-67. Featured article.
  53. 3D nuclear organization of telomeres in the Hodgkin cell lines U-HO1 and U-HO1-PTPN1: PTPN1 expression prevents the formation of very short telomeres including t-stumps. Knecht H, Bruderlein S, Wegener S, Lichtensztejn D, Lichtensztejn Z, Moller P and Mai S. BMC Cell Biology. 2010 Dec 14;11(1):99. [Epub ahead of print] BMC Cell Biology image of the month.
  54. Application of microarrays to the cytogenetic analysis of paediatric acute lymphoblastic leukemias. Dawson AJ, Yanofsky R, Vallente R, Bal S, Schroedter I, Liang L and Mai S. Current Oncology. 2010. In press.
  55. Homozygous BUB1B Mutation and Susceptibility to Gastrointestinal Neoplasia. Rio Frio T, Lavoie J, Hamel N, Geyer FC, Kushner YB, Novak DJ, Wark L, Capelli C, Reis-Filho JS, Mai S, Pastinen T, Tischkowitz MD, Marcus VA, Foulkes WD. N Engl J Med. 2010 Dec 30;363(27):2628-2637.
  56. Dynamic chromosomal rearrangements in Hodgkin's lymphoma are due to ongoing 3D nuclear remodeling and breakage-bridge-fusions. Guffei A, Sarkar R, Klewes R, Righolt C, Knecht H, Mai S. Haematologica. 2010 Dec;95(12):2038-46. Epub 2010 Sep 7.
  57. Duplication of sub-cytoband 11E2 of chromosome 11 is always associated with accelerated tumor development in v-abl/myc induced mouse plasmacytomas. Wiener F, Schmalter A-K, Mowat MRA, Mai S. Genes & Cancer 1(8): 847-858. 2010. Article and journal cover.
  58. Nuclear imaging in three dimensions: A unique tool in cancer research. Klonisch K, Wark L, Hombach-Klonisch S and Mai S. Ann Anat. 2010 Sep 20;192(5):292-301. Epub 2010 Aug 6. Article and journal cover.
  59. 3D structural and functional characterization of the transition from Hodgkin to Reed-Sternberg cells. Knecht H, Bruderlein S, Mai S, Moller P, Sawan B. Ann Anat. 2010 Sep 20;192(5):302-8. Epub 2010 Aug 6. Article and journal cover.
  60. p53 functions and cell lines: have we learned the lessons from the past? Millau J-F, Mai S, Bastien N, Drouin R. BioEssays 32: 392-400. 2010.
  61. Initiation of telomere-mediated chromosomal rearrangements in cancer. Prospects article. Mai S. J Cell Biochem. 109: 1095-1102. 2010. Featured article.
  62. Telomere-centromere-driven genomic instability contributes to karyotype evolution in a mouse model of melanoma. Silva AGdS, Graves HA, Guffei A, Ricca TI, Mortara, RA, Jasiulionis MG, Mai S. Neoplasia 12 (1): 11-19. 2010.
  63. Three-dimensional (3D) nuclear telomere architecture is associated with differential time to progression and overall survival in glioblastoma patients. Gadji M, Fortin D, Tsanaclis A-M, Garini Y, Katzir N, Wienburg Y, Yan J, Klewes L, Klonisch T, Drouin R*, Mai S*. Neoplasia. 12 (2): 183-191. 2010. (*co-corresponding authors).
  64. 3D Telomere FISH defines LMP1 expressing Reed-Sternberg Cells as End-Stage Cells with Telomere-poor Ghost Nuclei and very short Telomeres. Knecht H, Sawan B, Lichtensztejn Z, Lichtenstejn D, Mai S. Lab Invest. 2010 Apr;90(4):611-9. Epub 2010 Feb 8.


Patent Title Inventors
Method of monitoring genetic instability using 3D microscopy and analysis Sharareh Moshir, Tony Chuang, Sabine Mai, Yuval Garini
Methods of diagnosis or detection using 3D analysis Sabine Mai, William Foulkes, Bart Vermolen, Tony Chuang, Alice Chuang
Diagnostic methods for hematological disorders Sabine Mai, Hans Knecht, Macura Gadji, Ade Olujohungbe
Methods for Diagnosing Alzheimer's Disease Sabine Mai, Angeles Garcia
Methods for Characterizing and Isolating Circulating Tumor Cell Subpopulations Sabine Mai, Yvon Cayre, Janine Wechsler
Methods of detecting and monitoring cancer using 3D analysis of centromeres Sabine Mai, Yuval Garini, Rahul Sarkar, Bart Vermolen

RIOH gratefully acknowledges the generous support of

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